Earlier mentioned protocols were followed

Earlier mentioned protocols [1,2] were followed right from the extraction up to qualitative and quantitative analysis of diatoms. Diatoms were identified on the basis descriptions available in the literature [3]. Distribution patterns of diatoms have been displayed in Table 4. Photomicrographs of some diatoms can also been viewed in Fig. 2 (Supplementary) and Fig. 1.
Acknowledgements
Data The data was acquired as a preliminary study for the research article entitled “The feasibility of Forward-projected model-based Iterative Reconstruction SoluTion (FIRST) for coronary 320-row computed tomography angiography: a pilot study \" [1]. The dataset of this article provides objective and subjective evaluations of 2mm–4mm coronary artery phantom scanned with 10%, 20% and 30% reduced dose from AIDR3D group and reconstructed with FIRST with 3 to 6 boards on each sides of the phantom. Fig. 1 represents the CT image of the phantom and 4 ROIs used for objective evaluation. Tables 1–4 show objective image quality in standard deviation, and subjective scores for each diameter of coronary artery phantom. Table 1 represents data for 3 boards (representing patients weighing 40–49kg), Table 2 represents 4 boards (50–59kg), Table 3 represents 5 boards (60–69kg), and Table 4 represents 6 boards (70–79kg).
Experimental design, materials and methods
Data Progenitor cell content for CD34+/CD45dim, CD34+/CD133+/CD45dim, subsets in 300uL aliquots of anticoagulated blood were measured by flow cytometry. Triplicate aliquots of blood from each sample were analyzed at each time point, and the mean values for each time point for every subject were calculated to determine the order AGI-5198 of the progenitor cell content during storage (Fig. 1). The standardized mean values for the 0–4h time point was used as the baseline, and the relative change of mean values for the subsequent time points was calculated as a percentage of the baseline value. We found no significant differences in PC counts for both CD34+ (Fig. 1 Panel A, P=0.68) and CD34+/CD133+ (Fig. 1 Panel B, P=0.74).
Experimental design, materials and methods Gently mix by inversion and reverse pipet 300ul blood sample to a 5ml FACS tube. Add antibody cocktail to blood sample and vortex and incubate in the dark for 15min. Add 1.2ml Ammonium chloride lysis buffer, vortex and incubate in the dark for 10min. Sample should become relatively transparent post lysis. Add 1.2ml staining media the add 350ul 1% paraformaldehyde to fix cells, seal the tubes with parafilm and mix gently by inverting several times. Store samples at 4°C. Before acquisition on the FACS Canto II, reverse pipet 100ul Invitrogen counting beads to the prepared samples, mixed gently and run. FCS files we a’re analyzed in FlowJo version 9.8.5.
Materials
Data Fig. 1 reports the CD spectra of β-Lg with different concentrations of EGCg or Cu2+ or Al3+. The negative bands at 222nm could indicate the α-helix structure of the proteins [1,3].
Experimental design, materials and methods
Acknowledgements This work was supported by the Key Lab. of Biomass and Energy and Material Jiangsu Province (KLBEM) (JSBEM-S-201707) and National Key Research and Development Plan (2016YFD0600806).
Data The dataset of this article is the protocol achieved in Sánchez-Ramos et al. [1]
Experimental design, materials and methods
Acknowledgments This work was supported by Ministry of Health of Government of Andalusia, Grant number: PI-057/2012.
Data
Experimental design, materials and methods
Acknowledgements This research was supported by Australian Research Council grants to King (FT130101524 and DP150104604). The Centre for Advanced Microscopy is supported by the Australian Microscopy and Microanalysis Research Facility (AMMRF). We thank Lasse Noren from the Research School of Chemistry, Australian National University for assistance with the X-ray diffraction data collection.