br Experimental design materials and methods The

Experimental design, materials and methods The original map was obtained as a TIFF image from [1] and was imported to GlobalMapper v11.01 (Global Mapper Software LLC). The image was then combined with a basemap of South America and the limits of each province and subprovince were set and adjusted. Then, every polygon was assigned property fields with its corresponding name, subprovince, province and code as in [1]. The shapefile can be downloaded from https://figshare.com/s/c3135ce20c9ad8b7541a. This shapefile was entirely created using the criteria established in [1] to define different biogeographical areas of the southern-most portion of South America (Fig. 1). This area was also studied by [2], but the differences between both criteria are significant enough to validate the creation of a layer based on [1], so ecologists have the possibility of comparing the suitability of them to their subjects.
Acknowledgements The author would like to thank Juan José Morrone for providing the TIFF image that led the shapefile creation and Consejo Nacional de Investigaciones Científicas y Técnicas for funding the research.
1. Data The dataset of this article described the consequence of SiO2 nanoparticles in the synthesis of superabsorbent hydrogel polymer from the crosslinking in between carboxymethyl CC-10004 (CMC) and starch with aluminum sulfate. The Fig. 1 shows the polymer film formation and hydrogel, Fig. 2 shows the electrical conductivity property of polymer film, Fig. 3 shows the plant growth. Table 1 shows the water retention of hydrogel with differing concentration of aluminium sulfate and Table 2 shows the growth of the plant in the presence and absence of hydrogel polymer.
Experimental design, materials and methods: Synthesis of hydrogel polymer was conducted with SiO2 nanoparticles and analyzed for water retention capacity. Carboxymethyl cellulose sodium salt (CMC) and starch soluble in addition to SiO2 (20nm) nanoparticles (enhance the surface area) were used to synthesize superabsorbent polymer films and aluminum sulfate octadecahydrate was used to establish crosslink in between polymer composite [4,5].The DC electrical conductivities of polymer samples were analyzed by using 4200-SCS Keithley, it is a modular uses for characterization of electrical properties of materials. Two metallic electrodes were then connected to samples using silver wires. The transient I–V measurements done at room temperature. I–V characteristics help to determine electrical conductivities polymer samples. The amount of water retention was calculated by the formula (Gs−Gi)/Gi, where Gs is the weight of hydrogel after water absorption and Gi is the original weight of the superabsorbent polymer. Superabsorbent polymer was mixed with agriculture soil and used for sowing seeds of Vigna mungo[1–5]. Three replicates were used for the investigation of plants growth and germination of seeds.
Acknowledgments Authors are grateful to GKV, Haridwar for providing instrumental and other facilities. This work is funded by BSR section, University Grants Commission, New Delhi, India (No. F. 25-1/2013-14(BSR)/11-13/2008(BSR)). Under the scheme of UGC BSR research fellowship in sciences, bio-sciences, agriculture sciences, medical sciences and engineering Sciences to university/departments.
Data Rats were fed an enriched strawberry diet for two months and were weighed once a week for the whole experimental period. Compared with control group, strawberry supplementation didn’t interfere with body weight (Fig. 1) and liver ratio (Table 1). In addition, strawberry consumption didn’t affect biomarkers of liver injury measured in plasma (Table 1) as well as liver histology (Fig. 2): no differences were in fact observed for these parameters in the group supplemented with strawberries compared to the control group.
Experimental design Wistar rats (Rattus norvegicus, 19–21 months, 500–550g) provided by the “Istituto Nazionale di Ricovero e Cura per gli Anziani” (INRCA, Ancona, Italy), were housed individually and maintained on a 12h light/12h darkness cycle with free access to drinking water. The animals randomly received either a standard diet (C group, n=8) or a strawberry-enriched diet (S group, n=8) (Table 2) for 2 months. Both diets were supplied in the form of powder and daily prepared by mixing each individual ingredient using a rotating mixer and kept in the dark at a temperature of 4°C. Compared to the standard diet (AIN93M), the strawberry enriched diet was prepared by substituting 15% of the total calories with freeze-dried strawberry powder (Tables 2 and 3), and the amount of macro- and micronutrient adjusted to be identical between the two diets. The animals received their respective food and drink at libitum. Rats were weighed once a week for the whole experimental period.