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  • Funding from the German Federal Ministry of Education

    2018-10-29

    Funding from the German Federal Ministry of Education and Research (BMBF) supported parts of this study (Funding code FKZ 16I1619). The Leibniz Center for Agricultural Landscape Research (ZALF) is institutionally funded by the Federal Ministry of Food and Agriculture (BMEL) and the Ministry for Science, Research and Culture of the State of Brandenburg (MWFK).
    Data The data shared in this article is sterilization efficacy of benzalkonium chloride and callogenic response in presence of different PGRs. The significant difference in Tubastatin A manufacturer germination with the treatment using treatment code S1MS and S3MS even after 90 days of incubation indicates the adverse effects of HgCl2 on germination of C. carandas seeds (Fig. 1). This data also reveals that effect of PGRs treatment towards callogenic response and best response was recorded in media code A3 and B6 with friable callus (Fig. 2) while media code A4 gave embryogenic callus (Fig. 3).
    Experimental design, materials and methods Seeds of C. carandas are recalcitrant and having low viability, poor germination and they are most sensitive to chemical treatment [1,2]. Hence, these seeds were used to investigate the effect of various sterilants to remove surface born microorganism without any adverse effect on seeds.
    Acknowledgments
    Data Data shared with this article are more than 100 data of solubility and diffusivity of gases (O2 and CO2) in food samples. These data are stored in a data warehouse called @Web in which the data management is guided by ontology. All data are available for uploading at the URL specified below and recalled in the table hereafter with the details about the nature and amount of data available at each URL.
    Experimental design, materials and methods O. Oxygen optical sensors (Presens GmbH, Regensberg, Germany) were used to monitor O2 partial pressure. This measurement is based on dynamic luminescence quenching. Due to an excitation flash emitted through an optical fibre, the luminophore contained in the sensor goes into an excited state and thus emits fluorescence backscatter signal, which is detected by the optical fibre. If the luminophore is in contact with an oxygen molecule, the backscatter signal is changed due to a dynamic quenching of luminescence. The change in the backscatter signal permits to detect the O2 partial pressure in the medium. Two different set-ups exist (1) an invasive O2-sensitive optical sensor made of a syringe probe (micro-sensors, Presens GmbH, Regensburg, Germany) connected to the optical fibre and oxygen metre (Oxy-4 micro, Presens) and (2) a non-invasive oxygen sensor made of a dot of 5mm of diameter that can be stuck on the wall of a transparent container and measurement is then made through the transparent container. Oxygen sorption kinetics were measured at fixed temperature value when imposing a controlled partial pressure of O2 in the surrounding of the sample. The mono-directional O2 ingress into the sample was measured locally at the bottom or in the middle of the thin layer of food material previously free of O2 using one of the aforementioned sensors. More details on the experimental set-up could be found in [1–3]. CO. The solubility of CO2 was measured at equilibrium by quantification of the gas dissolved in the sample using chemical titration [4,5]. This measurement was done in a set-up where the sample is in a controlled chamber (controlled temperature, relative humidity, CO2 gas composition). The diffusion of CO2 was characterised by (1) imposing a gradient of CO2 to a piece of material of simple geometry (cylinder or plane sheet), (2) measuring the CO2 sorption kinetic in the sample and (3) identifying diffusivity values by adjusting a dedicated mathematical model to the experimental kinetic. Two types of kinetic could be obtained: (1) CO2 space-dependent profile in the cylindrical sample after its slicing and CO2 quantification in each slice or (2) CO2 time-dependent profile after CO2 quantification in each thin slice (one Tubastatin A manufacturer slice corresponding at one time of kinetic) [4,6].