Data experimental design materials and methods br Acknowledg

Data, experimental design, materials and methods
Acknowledgments This research was supported by funding from JSPS KAKENHI (22580136, 24658228 and 26712023); and Kyushu University Interdisciplinary Programs in Education and Projects in Research Development (P&P A-type, 25005).
Data, experimental design, materials and methods We have established a fluorescence-based assay for quantitative monitoring of DNA synthesis based on the formation of pyrophosphate [1]. The assay was developed for the use with DNA containing different DNA alkylation products. DNAs were analyzed with Mass Spectrometry and the corresponding spectra are shown in Supplementary materials. Replication of modified and natural DNA templates by Y-family translesion synthesis polymerases was monitored and quantified based on fluorescence emission being proportional to pyrophosphate release. The data were correlated to those produced by monitoring replication of modified and natural DNA templates via gel electrophoresis, and the comparative data are shown in Supplementary materials. The data support the reliability of the assay and its applicability to high throughput screening of polymerase inhibitors. Validation of the assay with unnatural nucleosides resulted in the observation that lesion-specific inhibition of translesion synthesis is possible.
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Data, experimental design, materials and methods
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Data, experimental design and methods
To investigate whether ATP4 was required for selective androgen receptor modulators cilia, we analyzed cerebrospinal fluid (CSF) flow as a proxy. For imaging and calculation of ependymal flow cf. Hagenlocher et al. [6] and Walentek et al. [18]. To facilitate late analysis of atp4a morphants, we used 1pmol/injection of a splice-site MO (atp4aSplMO) which targeted the second exon/intron boundary of zygotically expressed pre-mRNA. Injection of fluorescent beads into the brain ventricles at stage 45 revealed a significant reduction of CSF flow velocity in atp4a morphants (p<0.001; Fig. 3; Movie 1). In contrast, velocity of CSF flow in atp4a morphants was increased by co-injection of either atp4a or foxj1 DNA constructs (p<0.05/0.001; Fig. 3; Movie 1). The following is the Supplementary material related to this article Movie 1.
High levels of atp4a transcripts were found in the stomach of the tadpole (Fig. 5A and C), where ATP4 localization and function have been previously described [8]. We also observed weaker atp4a expression in the embryonic esophagus and the proximal small intestine (Fig. 5D). Analysis of foxj1 mRNA transcription in stage 45 tadpoles revealed expression in the very same regions of the gastrointestinal (GI) tract (Fig. 5E and F).
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Funding Work in the Blum lab was supported by a grant from the Deutsche Forschungsgemeinschaft (BL-285/9) to MB. Xenopus work in the Harland lab was supported by the National Institutes of Health (NIH) (GM42341). PW and TB were recipients of Ph.D. fellowships from the Landesgraduiertenförderung Baden-Württemberg. PW and open-access publication of this article were supported by a postdoctoral fellowship form the Deutsche Forschungsgemeinschaft (Wa 3365/1-1). CH and KF are indebted to the Baden-Württemberg Stiftung for the financial support of their research by the Eliteprogramme for Postdocs. KF was supported by a Margarete-von-Wrangell fellowship, funded by the European Social Fund and by the Ministry Of Science, Research and the Arts in Baden-Württemberg.
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Data, experimental design, materials and methods The data shown here are two lists of cellular and mitochondrial proteins found by FPLC, SDS-Page and mass spectrometry, that are differentially expressed in omental adipose tissue of obese and non-obese humans. Supplementary Table 1 contains a list of all 126 proteins found and includes also proteins of non-mitochondrial origin. Supplementary Table 2 contains all 62 mitochondrial proteins with potentially different abundance [1].