br Data The data being shared describe the mechanical and

Data The data being shared describe the mechanical and structural properties of healing rat myocardial infarcts from individual samples in several post-infarct time points: immediately (i.e. day), 7, 14 and 28 days after the induction of myocardial infarction. The mechanical data are composed of biaxial tensile stress–strain relationships (Figs. 1–4), biaxial tensile moduli (Table 1), compressive stress–strain relationships (Fig. 5) and compressive moduli (Table 2). The structural data include the angles of collagen fibres orientation measured at the centre of infarct samples (Table 3).
Experimental design, materials and methods The details of the experimental work and materials are provided in [1].
Acknowledgements The authors would like to thank Prof Jeffrey W. Holmes from Robert M. Berne Cardiovascular Research Center, University of Virginia, for his helpful and constructive comments that greatly contributed to improving the final version of the paper. The authors would like to also thank Mrs. Nancy Pounds and Mrs. Jamie Walker from the College of Veterinary Medicine, Mississippi State University, for the help with the anaesthesia and operation room management. This study was supported financially by the National Research Foundation (NRF) of South Africa (UID92531) and the Centre for High Performance Computing, Council for Scientific and Industrial Research, South Africa (UCTUO18697). Any opinion, findings and conclusions or recommendations expressed in this cyclin dependent kinases publication are those of the authors and therefore the NRF does not accept any liability in regard thereto. MSS acknowledges the International Society of Biomechanics Matching Dissertation Grant. NHD acknowledges financial support from the South African Medical Research Council.
Data 15 SSR markers were developed and tested on selected Garcinia mangostana accessions (Table 1) [1]. The data presented here shows that the ISSR-Suppression-PCR technique was very applicable in designing SSR primers and was able to reveal variation of selected germplasm collections [2,3]. These SSR markers can be used to assess genetic variation of G. mangostana.
Experimental design, materials and methods
Acknowledgements This work was supported by Grant UKM-AP-KPB-18-2010 from the Ministry of Higher Education, Malaysia.
Specifications Table Value of the data Data The data being shared consists in NMR spectra, as well as high-resolution mass spectrometry spectra and gas chromatography–mass spectrometry chromatograms used to identify fatty acid esters from the phytopathogenic fungus, L. theobromae. Other fungi such as Neofusicoccum parvum, Fusarium oxysporum f.sp. lycopersici and Trichoderma asperellum were also studied for comparison. Images of mycelial morphology for L. theobromae in different carbon sources are shown. Effects of fatty acid esters produced by L. theobromae in N. tabacum morphology are included. Concentration gradients for the most physiologically active compounds, ethyl stearate (SAEE) and ethyl linoleate (LAEE) are also shown.
Experimental design, materials and methods For purification, mass spectrometry and nuclear magnetic resonance (NMR) [2,3], a modified Folch extraction [4] was standardized as described in [1], (Supplementary Data Set A and Fig. 1A, B). Carbon source effects were then studied in L. theobromae using the standardized Folch extraction. Fatty acid ester production was studied in the other fungal species N. parvum, F. oxysporum and T. asperellum for comparison. All samples, including the positive controls, were analyzed for naturally produced fatty acid ethyl esters by gas chromatography/mass spectrometry (GC–MS) as described in [1] (Figs. 2–5). The data was expressed as percent yield of each compound from the total compounds identified (Table 1). Morphology of L. theobromae incubated in 5% glucose was documented by photography and compared to the morphology in 5% glucose+5% grapeseed oil (Fig. 6). With the aim to test the effect of the isolated compounds in planta, we chose tobacco (Nicotiana tabacum), a well-studied plant model [5] to measure growth as described in [1]. The length of the seedling was measured after 7–10 days post-sowing using calibrated Image J software [6] from cotyledon tip to root tip for each experimental condition. Morphology was also assessed and documented 45 days post-dosing and sowing (Figs. 7–8). A high concentration gradient for the most physiologically active fatty acid esters found and described in [1] was performed by germinating the plant model N. tabacum in SAEE from 100 to 3.1μg/mL and LAEE from 200 to 3.1μg/mL (Fig. 9).